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A new apparatus called Bypass Trapped Flow Analysis System (ByT-FAS) is described. A properly designed ByT-FAS gives an analyst the ability to use analyte sample volumes of 10 to 200 μL [or more] and reagent volumes of approximately the same size. The sample and reagent are injected into their respective carrier streams and attain physical steady state concentrations in the detection cell within approximately 15 to 45 seconds after injection. Upon achievement of simultaneous sample and reagent physical steady state concentrations, the system flow is diverted around the detection cell and the reaction mixture is trapped in the detection cell. The concentration of the sample and reagent in the detection cell can be readily computed from knowledge of the original concentrations of the sample and reagent and the flow rates of the streams ...