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El objetivo del presente estudio ha sido evaluar la relación entre los parámetros de la calidad seminal y la capacidad de penetración in vitro de ovocitos homólogos (PIVh) con los resultados in vivo de fertilidad y prolificidad. Se han analizado 60 eyaculados realizando sobre cada uno las mediciones clásicas del espermiograma (motilidad, calidad de movimiento, volumen, concentración espermática, estado del acrosoma, morfoanomalías y tinción vital), pruebas bioquímicas (contenido de ATP, calcio, sodio, potasio, magnesio y zinc), ensayos de la funcionalidad del espermatozoide ( test de endósmosis, test del diacetato de carboxifluoresceína, test ORT) y la valoración mediante un test de penetración in vitro homóloga. Los resultados de la valoración in vitro del semen fueron contrastados con los resultados obtenidos en un ensayo in vivo...
This article analyses Miguel de Unamuno’s response to the Great War of 1914-1918 and the influence that the War had on Unamuno’s work and role as an intellectual. It deals with the following themes: the origins and nature of Unamuno’s support for the Allies during the War; the role that the War played in Unamuno’s evolution as an intellectual and political figure; Unamuno’s reading of the political situation in Spain over these years; and his understanding of the lasting effects and consequences of the War in Spain and in Europe as a whole.
Identificación de la empresa familiar desde el Derecho. Ubicación en la teoría jurídica de la empresa. Relaciones entre empresa familiar y Derecho de sociedades.
Animal models of human EBV-associated diseases are essential to elucidate the pathogenesis of EBV-associated diseases. Here we review those previous models using EBV or EBV-like herpesviruses and describe the details on our two newly-developed rabbit models of lymphoproliferative diseases (LPD) induced by simian EBV-like viruses. The first is Cynomolgus- EBV-induced T-cell lymphomas in rabbits inoculated intravenously (77- 90%) and orally (82- 89%) during 2 - 5 months. EBV-DNA was detected in peripheral blood by PCR from 2 days after oral inoculation, while anti- EBV-VCA IgG was raised 3 weeks later. Rabbit lymphomas and their cell lines contained EBV-DNA and expressed EBV-encoded RNA-1 (EBER-1). Rabbit lymphoma cell lines, most of which have specific chromosomal abnormality, showed tumorigenicity in nude mice. The seco...
The present study reported histochemical changes in alveolar bone glycosaminoglycans (GAG) (using Safranin 0 ) and in interdental bone height in three groups of BB/W rats: diabetic, diabetes prone, and diabetes resistant. Safranin O staining intensity suggested that total GAG levels were highest in diabetic bone (p<0.05 compared to diabetes resistant, p<0.005 compared to diabetes prone) but not significantly different between diabetes prone and resistant groups. Following chondroitinase AC and ABC digestion, staining reactions suggested that the highest levels of dermatan sulfate were in the diabetes resistant group (p<0.001 compared to diabetic, p<0.001 compared to diabetes prone) and the highest levels of chondroitin sulfates were in the diabetes prone group (p<0.001). Coincidently the mean height of diabetes prone in...
Substance P-immunoreactive (SP-I) structures in the carotid bodies of rats and cats were examined with the light and electron microscopes. In both species SP-I varicose nerve fibers were located singly in the interstitial connective tissue in close association with blood vessels. They were small unmyelinated fibers enveloped in a common Schwann cell sheath with other SP-negative fibers. Some of SP-I fibers contained large dense-cored granules and small clear vesicles in addition to microtubules and mitochondria and probably represented nerve fiber varicosities. The latter often were found incompletely invested by Schwann cell sheaths. SP-fibers were found occasionally in the envelopes of supporting cells at the periphery of parenchymal cell groups. However, none of the nerve terminals making synaptic contacts with glomus ...